The Netherlands international suffered the injury during Barcelona's training session ahead of their weekend La Liga game against Villarreal. Initial tests suggest a possible muscle tear, which could keep him out for four weeks.
De Jong's absence means he will miss several key matches including the Copa del Rey semi-final second leg vs Atletico Madrid, the Champions League Round of 16 tie, and multiple La Liga fixtures. The injury comes as a significant blow to manager Hansi Flick, who had just about a fully fit squad available.